Composite
Part:BBa_K634007:Experience
Designed by: Eric Walters Group: iGEM11_Wisconsin-Madison (2011-09-23)
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how you used this part and how it worked out.
Issues in practice
sacB appears to be something of a lightning rod for loss of function mutations. As can be seen above, sucrose lethality is only maintained in a population for approximately 5 hours. At this point, we presume that spontaneous mutations in sacB produce sucrose-insensitive mutants.
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