Composite

Part:BBa_K634007:Experience

Designed by: Eric Walters   Group: iGEM11_Wisconsin-Madison   (2011-09-23)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.


Issues in practice

sacB appears to be something of a lightning rod for loss of function mutations. As can be seen above, sucrose lethality is only maintained in a population for approximately 5 hours. At this point, we presume that spontaneous mutations in sacB produce sucrose-insensitive mutants.

User Reviews

UNIQ8203f732825cb5b8-partinfo-00000000-QINU UNIQ8203f732825cb5b8-partinfo-00000001-QINU