Composite

Part:BBa_K634007:Experience

Designed by: Eric Walters   Group: iGEM11_Wisconsin-Madison   (2011-09-23)

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Issues in practice

sacB appears to be something of a lightning rod for loss of function mutations. As can be seen above, sucrose lethality is only maintained in a population for approximately 5 hours. At this point, we presume that spontaneous mutations in sacB produce sucrose-insensitive mutants.

User Reviews

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